The effect of six weeks of sour cherry juice consumption with combined exercises on AST, ALT, TAC and MDA in obese and overweight women

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.

Abstract

Aim: Obesity is associated with increased oxidative stress and chronic inflammation, which can negatively affect liver function; therefore, nutritional and exercise interventions have been considered as effective strategies to improve this condition. Consumption of tart cherry juice, due to its antioxidant and anti-inflammatory compounds, along with combined exercises may have positive effects on liver and oxidative markers in overweight and obese women.

Method: This quasi-experimental study was conducted with a pretest-posttest design in two groups: combined exercise and combined exercise with cherry juice supplementation, on 24 obese or overweight women aged 20-35 years. The intervention consisted of six weeks of combined exercise (resistance and aerobic) in three 90-minute sessions per week. The supplementation group consumed 240 ml of cherry juice daily. Data were analyzed with a covariance test for between-group comparisons and a paired t-test for within-group changes at a significance level of P≥0.05.

Results: The results of the covariance test showed that after six weeks of intervention, there was no significant difference between the two groups in AST, ALT, TAC, and MDA values (P<0.05).

Conclusion: According to the results, consuming tart cherry juice supplements along with combined exercises for six weeks did not have a significant effect on inflammatory and oxidative indices, although the trend of changes indicated the potential synergistic effects of this intervention.

Highlights

The present study showed that six weeks of tart cherry juice consumption combined with concurrent training induced changes in inflammatory and oxidative stress indices, although these changes were not statistically significant. The effectiveness of this combined strategy appears to depend on factors such as dose, intervention duration, and baseline participant characteristics. Future studies should examine different doses, longer intervention periods, and specific populations such as women with obesity to provide more definitive evidence regarding the effects of this intervention.

Keywords

Main Subjects


  • [1] Kivimäki M, Strandberg T, Pentti J, Nyberg ST, Frank P, Jokela M, et al. Body-mass index and risk of obesity-related complex multimorbidity: an observational multicohort study. The Lancet Diabetes & Endocrinology. 2022;10(4):253-263, Doi: https://doi.org/10.1016/S2213-8587(22)00033-X
  • [2] Chavarría-Arciniega S, López-Alvarenga JC, Uribe-Uribe NO, Herrera-Hernández M, González-Barranco J. Relationship between morphological diagnosis of NASH (Non-Alcoholic SteatoHepatitis) and liver function test in a group of patients with morbid obesity. Revista de Investigación Clínica. 2005;57(4):505-512.
  • [3] Whitfield JB. Gamma glutamyl transferase. Critical Reviews in Clinical Laboratory Sciences. 2001;38(4):263-355, Doi: https://doi.org/10.1080/20014091084227
  • [4] Purcell M, Flores YN, Zhang ZF, Denova-Gutiérrez E, Salmeron J. Prevalence and predictors of alanine aminotransferase elevation among normal weight, overweight and obese youth in Mexico. Journal of Digestive Diseases. 2013;14(9):491-499, Doi: https://doi.org/10.1111/1751-2980.12072
  • [5] Cruz MAF, Cruz JF, Macena LB, de Santana DS, da Cunha Oliveira CC, Lima SO, et al. Association of the nonalcoholic hepatic steatosis and its degrees with the values of liver enzymes and homeostasis model assessment-insulin resistance index. Gastroenterology Research. 2015;8(5):260, Doi: https://doi.org/10.14740/gr685w
  • [6] Schindhelm RK, Diamant M, Dekker JM, Tushuizen ME, Teerlink T, Heine RJ. Alanine aminotransferase as a marker of non-alcoholic fatty liver disease in relation to type 2 diabetes mellitus and cardiovascular disease. Diabetes/Metabolism Research and Reviews. 2006;22(6):437-443, Doi: https://doi.org/10.1002/dmrr.666
  • [7] Wang D, Zhou B-Y, Xiang L, Chen X-Y, Feng J-X. Alanine aminotransferase as a risk marker for new-onset metabolic dysfunction-associated fatty liver disease. World Journal of Gastroenterology. 2024;30(25):3132, Doi: https://doi.org/10.3748/wjg.v30.i25.3132
  • [8] Vozarova B, Stefan N, Lindsay RS, Saremi A, Pratley RE, Bogardus C, et al. High alanine aminotransferase is associated with decreased hepatic insulin sensitivity and predicts the development of type 2 diabetes. Diabetes. 2002;51(6):1889-1895, Doi: https://doi.org/10.2337/diabetes.51.6.1889
  • [9] Nikoo M, Ebrahimi M. Assessment of changes in energy intake and appetite in response to exercise during fasting and after breakfast in overweight women. Research in Exercise Nutrition. 2024;3(2):22-13, Doi: https://doi.org/10.22034/ren.2025.143059.1073
  • [10] Lee D-H, Jacobs DR Jr, Gross M, Kiefe CI, Roseman J, Lewis CE, et al. γ-Glutamyltransferase is a predictor of incident diabetes and hypertension: the Coronary Artery Risk Development in Young Adults (CARDIA) Study. Clinical Chemistry. 2003;49(8):1358-1366, Doi: https://doi.org/10.1373/49.8.1358
  • [11] Nannipieri M, Gonzales C, Baldi S, Posadas R, Williams K, Haffner SM, et al. Liver enzymes, the metabolic syndrome, and incident diabetes: the Mexico City Diabetes Study. Diabetes Care. 2005;28(7):1757-1762, Doi: https://doi.org/10.2337/diacare.28.7.1757
  • [12] Li M, Zhang S, Wu Y, Ye J, Cao X, Liu J, et al. Prevalence of insulin resistance in subjects with nonalcoholic fatty liver disease and its predictors in a Chinese population. Digestive Diseases and Sciences. 2015;60:2170-2176, Doi: https://doi.org/10.1007/s10620-015-3564-5
  • [13] Liu C, Shao M, Lu L, Zhao C, Qiu L, Liu Z. Obesity, insulin resistance and their interaction on liver enzymes. PLoS One. 2021;16(4):e0249299, Doi: https://doi.org/10.1371/journal.pone.0249299
  • [14] Bennett JM, Reeves G, Billman GE, Sturmberg JP. Inflammation—nature's way to efficiently respond to all types of challenges: implications for understanding and managing ‘the epidemic’ of chronic diseases. Frontiers in Medicine. 2018;5:316, Doi: https://doi.org/10.3389/fmed.2018.00316
  • [15] Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2017;9(6):7204, Doi: https://doi.org/10.18632/oncotarget.23208
  • [16] Keshavarz F, Anoushirvani S, Arazi H, Afroundeh R, Golpasandi H. The effect of HIFT with ginger extract supplementation on the levels of some antioxidant enzymes in men with non-alcoholic fatty liver. Research in Exercise Nutrition. 2025;3(3):12-1, Doi: https://doi.org/10.22034/ren.2025.143126.1081
  • [17] Ilbeigi S, Torab beygi Z, Saghebjoo M, Jafari M. Investigating the Effect of Two-Week L-Carnitine Supplementation on Serum Levels of Total Antioxidant Capacity and Malondialdehyde Following Intense Aerobic Exercise in Female Karate Athletes. Research in Exercise Nutrition. 2024;3(4):23-13, Doi: https://doi.org/10.22034/ren.2025.143618.1090 .
  • [18] Krzemińska J, Wronka M, Młynarska E, Franczyk B, Rysz J. Arterial hypertension—oxidative stress and inflammation. Antioxidants. 2022;11(1):172, Doi: https://doi.org/10.3390/antiox11010172
  • [19] Zhang Z, Zhao L, Zhou X, Meng X, Zhou X. Role of inflammation, immunity, and oxidative stress in hypertension: new insights and potential therapeutic targets. Frontiers in Immunology. 2023;13:1098725, Doi: https://doi.org/10.3389/fimmu.2022.1098725
  • [20] Cuevas-Cianca SI, Romero-Castillo C, Gálvez-Romero JL, Juárez ZN, Hernández LR. Antioxidant and anti-inflammatory compounds from edible plants with anti-cancer activity and their potential use as drugs. Molecules. 2023;28(3):1488, doi: https://doi.org/10.3390/molecules28031488
  • [21] Ravipati AS, Zhang L, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, et al. Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complementary and Alternative Medicine. 2012;12:1-14, doi: https://doi.org/10.1186/1472-6882-12-173
  • [22] Blando F, Oomah BD. Sweet and sour cherries: origin, distribution, nutritional composition and health benefits. Trends in Food Science & Technology. 2019;86:517-529, doi: https://doi.org/10.1016/j.tifs.2019.02.052
  • [23] Kelley DS, Adkins Y, Laugero KD. A review of the health benefits of cherries. Nutrients. 2018;10(3):368, doi: https://doi.org/10.3390/nu10030368
  • [24] Escribano BM, Moreno A, Tasset I, Túnez I. Impact of light/dark cycle patterns on oxidative stress in an adriamycin-induced nephropathy model in rats. PLoS One. 2014;9(5):e97713, doi: https://doi.org/10.1371/journal.pone.0097713
  • [25] de Sousa CV, Sales MM, Rosa TS, Lewis JE, de Andrade RV, Simões HG. The antioxidant effect of exercise: a systematic review and meta-analysis. Sports Medicine. 2017;47:277-293, doi: https://doi.org/10.1007/s40279-016-0566-1
  • [26] Gomez-Cabrera M-C, Domenech E, Viña J. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radical Biology and Medicine. 2008;44(2):126-131, Doi: https://doi.org/10.1016/j.freeradbiomed.2007.02.001
  • [27] Levers K, Dalton R, Galvan E, Goodenough C, O'Connor A, Simbo S, et al. Effects of powdered Montmorency tart cherry supplementation on an acute bout of intense lower body strength exercise in resistance-trained males. Journal of the International Society of Sports Nutrition. 2015;12(1):41, doi: https://doi.org/10.1186/s12970-015-0102-y
  • [28] Hillman AR, Trickett O, Brodsky C, Chrismas B. Montmorency tart cherry supplementation does not impact sleep, body composition, cellular health, or blood pressure in healthy adults. Nutrition and Health. 2022:02601060221111230, Doi: https://doi.org/10.1177/02601060221111230
  • [29] Johnson SA, Navaei N, Pourafshar S, Jaime SJ, Akhavan NS, Alvarez-Alvarado S, et al. Effects of Montmorency tart cherry juice consumption on cardiometabolic biomarkers in adults with metabolic syndrome: a randomized controlled pilot trial. Journal of Medicinal Food. 2020;23(12):1238-1247, Doi: https://doi.org/10.1089/jmf.2019.0240
  • [30] Martin KR, Bopp J, Neupane S, Vega-Lopez S. 100% tart cherry juice reduces plasma triglycerides and CVD risk in overweight and obese subjects. The FASEB Journal. 2010;24:722.14-.14, doi: https://doi.org/10.1096/fasebj.24.1_supplement.722.14
  • [31] Martin KR, Burrell L, Bopp J. Authentic tart cherry juice reduces markers of inflammation in overweight and obese subjects: a randomized, crossover pilot study. Food & Function. 2018;9(10):5290-5300, Doi: https://doi.org/10.1039/c8fo01492b
  • [32] Nemes A, Homoki JR, Kiss R, Hegedűs C, Kovács D, Peitl B, et al. Effect of anthocyanin-rich tart cherry extract on inflammatory mediators and adipokines involved in type 2 diabetes in a high-fat diet-induced obesity mouse model. Nutrients. 2019;11(9):1966, Doi: https://doi.org/10.3390/nu11091966
  • [33] Martinelli I, Tomassoni D, Bellitto V, Roy P, Micioni Di Bonaventura MV, Amenta F, et al. Anti-inflammatory and antioxidant properties of tart cherry consumption in the heart of obese rats. Biology. 2022;11(5):646, Doi: https://doi.org/10.3390/biology11050646
  • [34] Moruzzi M, Klöting N, Blüher M, Martinelli I, Tayebati SK, Gabrielli MG, et al. Tart cherry juice and seeds affect pro-inflammatory markers in visceral adipose tissue of high-fat diet obese rats. Molecules. 2021;26(5):1403, Doi: https://doi.org/10.3390/molecules26051403
  • [35] Kimble R, Keane KM, Lodge JK, Howatson G. The influence of tart cherry (Prunus cerasus, cv Montmorency) concentrate supplementation for 3 months on cardiometabolic risk factors in middle-aged adults: a randomised, placebo-controlled trial. Nutrients. 2021;13(5):1417, Doi: https://doi.org/10.3390/nu13051417
  • [36] Dehghani E, Beba M, Danandeh K, Memari A, Ershadmanesh MJ, Rasoulian P, et al. The effect of tart cherry juice (TCJ) supplementation on exercise-induced muscle damage (EIMD) in an athletic population. Annals of Medicine and Surgery. 2025;87(2):880-890, Doi: https://doi.org/10.1097/ms9.0000000000002914
  • [37] McHugh MP. ‘Precovery’ versus recovery: understanding the role of cherry juice in exercise recovery. Scandinavian Journal of Medicine & Science in Sports. 2022;32(6):940-950, Doi: https://doi.org/10.1111/sms.14141
  • [38] Anyanwu CF, Aigbogun EO Jr, Joseph TO. Evaluation of the liver enzyme (AST, ALT & ALP) levels of adult HIV patients on HAART in UPTH. Annual Research & Review in Biology. 2020;35:34-41.
  • [39] de Sousa BRV, de Lima Tavares Toscano L, de Almeida Filho EJB, Sena KF, Costa MS, de Souza Cunha RC, et al. Purple grape juice improves performance of recreational runners, but the effect is genotype dependent: a double-blind, randomized, controlled trial. Genes & Nutrition. 2022;17(1):9, Doi: https://doi.org/10.1186/s12263-022-00710-1
  • [40] Chai SC, Davis K, Zhang Z, Zha L, Kirschner KF. Effects of tart cherry juice on biomarkers of inflammation and oxidative stress in older adults. Nutrients. 2019;11(2):228, Doi: https://doi.org/10.3390/nu11020228
  • [41] Moayedi F, Taghian F, Dehkordi KJ, Hosseini SA. Cumulative effects of exercise training and consumption of propolis on managing diabetic dyslipidemia in adult women: a single-blind, randomized, controlled trial with pre-post-intervention assessments. The Journal of Physiological Sciences. 2023;73(1):17, Doi: https://doi.org/10.1186/s12576-023-00872-6

 

  • Receive Date: 06 June 2025
  • Revise Date: 20 August 2025
  • Accept Date: 26 August 2025
  • First Publish Date: 26 August 2025